1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response

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	1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response
	Huang, Yan-Ping 1,2,3; He, Tao-Bin 1,2,3,4; Cuan, Xian-Dan 1,2,3; Wang, Xuan-Jun 1,2,3; Hu, Jiang-Miao4 ; Sheng, Jun 1,2,3
	2018-10-01
发表期刊	MOLECULES
ISSN	1420-3049
卷号	23 期号:10 页码:15
摘要	2,3-O-acetylated-1,4-beta-D-glucomannan (DOP-1-1) is a polysaccharide isolated from the stem of Dendrobium officinale. DOP-1-1 has been demonstrated to have remarkable immunomodulatory properties, but little is known about the influence of its structural diversity on bioactivity (and even less about the exact mechanism underlying its immune responses). First, DOP-1-1 was stabilized at different temperatures and pH conditions based on differential scanning calorimetry and size exclusion-chromatography-high-performance liquid chromatography. Then, a detailed study on the effects of DOP-1-1 on a human leukemia monocytic cell line (THP-1) under normal conditions was undertaken. DOP-1-1 promoted the translocation of nuclear factor-kappa B (NF-kappa B) and degradation of I kappa B proteins. The expression of genes and proteins closely associated with the immune, survival and apoptotic functions of NF-kappa B were analyzed by quantitative real-time RT-PCR. Furthermore, CCL4 and IP10 were confirmed to be the novel targets of the immune response stimulated by DOP-1-1. The phosphorylation of NF-kappa B was inhibited by treatment with a toll-like receptor 4 (TLR4) antagonist (TAK-242) and myeloid differentiation factor 88 (MyD88) inhibitor (ST2825). These data suggested: (i) the O-acetylated glucomannan DOP-1-1 is present in the steady state in low-pH solutions; (ii) DOP-1-1 can induce an immune response through NF-kappa B mediated by a TLR4 signaling pathway; and (iii) CCL4 and IP10 could be the novel targets of the immune response stimulated by O-acetylated glucomannan.
关键词	Dendrobium officinale 1,4-beta-D-glucomannan thermal stability NF-kappa B TLR4
收录类别	SCI
语种	英语
WOS记录号	WOS:000451201400254
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/63300
专题	中国科学院昆明植物研究所
通讯作者	Wang, Xuan-Jun; Hu, Jiang-Miao; Sheng, Jun
作者单位	1.Yunnan Agr Univ, Minist Educ, Key Lab Pu Er Tea Sci, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Sci, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yan-Ping,He, Tao-Bin,Cuan, Xian-Dan,et al. 1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response[J]. MOLECULES,2018,23(10):15.
APA	Huang, Yan-Ping,He, Tao-Bin,Cuan, Xian-Dan,Wang, Xuan-Jun,Hu, Jiang-Miao,&Sheng, Jun.(2018).1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response.MOLECULES,23(10),15.
MLA	Huang, Yan-Ping,et al."1,4-beta-D-Glucomannan from Dendrobium officinale Activates NF-kappa B via TLR4 to Regulate the Immune Response".MOLECULES 23.10(2018):15.