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DNA barcoding herbaceous and woody plant species at a subalpine forest dynamics plot in Southwest China
Tan, Shao-Lin1,2,3; Luo, Ya-Huang1; Hollingsworth, Peter M.4; Burgess, Kevin S.5; Xu, Kun6; Li, De-Zhu2,3; Gao, Lian-Ming1,6
2018-07-01
Source PublicationECOLOGY AND EVOLUTION
ISSN2045-7758
Volume8Issue:14Pages:7195-7205
Abstract

Although DNA barcoding has been widely used to identify plant species composition in temperate and tropical ecosystems, relatively few studies have used DNA barcodes to document both herbaceous and woody components of forest plot. A total of 201 species (72 woody species and 129 herbaceous species) representing 135 genera distributed across 64 families of seed plants were collected in a 25ha CForBio subalpine forest dynamics plot. In total, 491 specimens were screened for three DNA regions of the chloroplast genome (rbcL, matK, and trnH-psbA) as well as the internal transcribed spacers (ITS) of nuclear ribosomal DNA. We quantified species resolution for each barcode separately or in combination using a ML tree-based method. Amplification and sequencing success were highest for rbcL, followed by trnH-psbA, which performed better than ITS and matK. The rbcL+ITS barcode had slightly higher species resolution rates (88.60%) compared with rbcL+matK (86.60%) and rbcL+trnH-psbA (86.01%). The addition of trnH-psbA or ITS to the rbcL+matK barcode only marginally increased species resolution rates, although in combination the four barcodes had the highest discriminatory power (90.21%). The situations where DNA barcodes did not discriminate among species were typically associated with higher numbers of co-occurring con-generic species. In addition, herbaceous species were much better resolved than woody species. Our study represents one of the first applications of DNA barcodes in a subalpine forest dynamics plot and contributes to our understanding of patterns of genetic divergence among woody and herbaceous plant species.

KeywordDna Barcoding Forest Dynamics Plot Herbaceous Species Species Identification Woody Species Yulong Mountain
DOI10.1002/ece3.4254
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectEcology ; Evolutionary Biology
WOS IDWOS:000440138400031
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/62151
Collection中国科学院昆明植物研究所
Corresponding AuthorLi, De-Zhu; Gao, Lian-Ming
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China
3.Univ Chinese Acad Sci, Coll Life Sci, Kunming, Yunnan, Peoples R China
4.Royal Bot Garden Edinburgh, Edinburgh, Midlothian, Scotland
5.Univ Syst Georgia, Columbus State Univ, Dept Biol, Coll Letters & Sci, Columbus, GA USA
6.Chinese Acad Sci, Kunming Inst Bot, Lijiang Forest Ecosyst Res Stn, Lijiang, Peoples R China
Recommended Citation
GB/T 7714
Tan, Shao-Lin,Luo, Ya-Huang,Hollingsworth, Peter M.,et al. DNA barcoding herbaceous and woody plant species at a subalpine forest dynamics plot in Southwest China[J]. ECOLOGY AND EVOLUTION,2018,8(14):7195-7205.
APA Tan, Shao-Lin.,Luo, Ya-Huang.,Hollingsworth, Peter M..,Burgess, Kevin S..,Xu, Kun.,...&Gao, Lian-Ming.(2018).DNA barcoding herbaceous and woody plant species at a subalpine forest dynamics plot in Southwest China.ECOLOGY AND EVOLUTION,8(14),7195-7205.
MLA Tan, Shao-Lin,et al."DNA barcoding herbaceous and woody plant species at a subalpine forest dynamics plot in Southwest China".ECOLOGY AND EVOLUTION 8.14(2018):7195-7205.
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