Profiling membrane glycerolipids during γ-ray-induced membrane injury

doi:10.1186/s12870-017-1153-9

KIB OpenIR

	Profiling membrane glycerolipids during γ-ray-induced membrane injury
	Zheng,Guowei 1,2; Li,Weiqi1,2
	2017-11-15
发表期刊	BMC Plant Biology
ISSN	1471-2229
卷号	17 期号:1
摘要	AbstractBackgroundγ-rays are high-energy radiation that cause a range of random injuries to plant cells. Most studies on this issue have focused on γ-ray-induced nucleotide damage and the production of reactive oxygen species in cells, so little is known about the glycerolipid metabolism during γ-rays induced membrane injury. Using an ESI-MS/MS-based lipidomic method, we analysed the lipidome changes in wild-type and phospholipase D (PLD)δ- and α1-deficient Arabidopsis after γ-ray treatment. The aim of this study was to investigate the role of PLD-mediated glycerolipid metabolism in γ-ray-induced membrane injury.ResultsThe ion leakage of Arabidopsis leaves after 2885-Gy γ-ray treatment was less than 10%. High does γ-ray treatment could induce the accumulation of intracellular reactive oxygen species (ROS). Inhibition of PLDα1 caused severe lipid degradation under γ-ray treatment. γ-ray-induced glycerolipid degradation mostly happened in chloroplastidic lipids, rather than extraplastidic ones. The levels of lysophosphatidylcholine (lysoPC) and lysophosphatidylethanolamine (lysoPE) were maintained in the WS ecotypes during γ-ray treatments, while increased significantly in the Col ecotype treated with 1100?Gy. After 210- and 1100-Gy γ-ray treatments, the level of lysophosphatidylglycerol (lysoPG) decreased significantly in the four genotypes of Arabidopsis.Conclusionsγ-ray-induced membrane injury may occur via an indirect mechanism. The degradation of distinct lipids is not synchronous, and that interconversions among lipids can occur. During γ-ray-induced membrane injury, the degradation of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) may be mediated by PLDζ1 or phospholipase A1. The degradation of phosphatidylglycerol was not mediated by PLA, PLDδ or PLDα1, but by phospholipase C or other PLDs. γ-rays can decrease the double-bond index and increase the acyl chain length in membrane lipids, which may make membranes more rigid and further cause injury in membranes.
关键词	Gamma irradiation Membrane injury Phospholipase D Lipidomics Plastidic lipids Extraplastidic lipids
DOI	10.1186/s12870-017-1153-9
语种	英语
WOS记录号	BMC:10.1186/s12870-017-1153-9
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/61837
专题	中国科学院昆明植物研究所
通讯作者	Li,Weiqi
作者单位	1. 2.
推荐引用方式 GB/T 7714	Zheng,Guowei,Li,Weiqi. Profiling membrane glycerolipids during γ-ray-induced membrane injury[J]. BMC Plant Biology,2017,17(1).
APA	Zheng,Guowei,&Li,Weiqi.(2017).Profiling membrane glycerolipids during γ-ray-induced membrane injury.BMC Plant Biology,17(1).
MLA	Zheng,Guowei,et al."Profiling membrane glycerolipids during γ-ray-induced membrane injury".BMC Plant Biology 17.1(2017).