Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance
Chen, Qian; Yang, Shihai; Kong, Xiangxiang; Wang, Chuntao; Xiang, Nan; Yang, Yunqiang1; Yang, Yongping1
2018-07-30
Source PublicationGENE
ISSN0378-1119
Volume665Pages:41-48
Abstract

Stipa purpurea is widely distributed on the Tibetan Plateau, and has high drought resistance. Plasma membrane intrinsic proteins are a type of aquaporin. They regulate the movement of water and are associated with plant protective reactions to biotic and abiotic stresses. We characterized a plasma membrane intrinsic protein from S. purpurea (SpPIP1) and elucidated its role in molecular aspects of the plant's response to drought stress. The full-length open reading frame of SpPIP1 was 870 bp and encoded 289 amino acids. The transcript level of SpPIP1 was higher in the root of S. purpurea than in the flower, leaf and stem. The level of SpPIP1 transcript increased significantly when treated with drought treatment. Subcellular localization result showed that SpPIP1 was localized in the plasma membrane. Ectopic expression of SpPIP1 in Arabidopsis thaliana resulted in plants with higher tolerance to drought treatment. SpPIP1 of S. purpurea may mediate plant response to arid environments.

KeywordStipa Purpurea Pip1 Cloning Drought
DOI10.1016/j.gene.2018.04.056
WOS IDWOS:000435621900006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/61428
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Inst Tibetan Plateau Res Kunming, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Qian,Yang, Shihai,Kong, Xiangxiang,et al. Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance[J]. GENE,2018,665:41-48.
APA Chen, Qian.,Yang, Shihai.,Kong, Xiangxiang.,Wang, Chuntao.,Xiang, Nan.,...&Yang, Yongping.(2018).Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance.GENE,665,41-48.
MLA Chen, Qian,et al."Molecular cloning of a plasma membrane aquaporin in Stipa purpurea, and exploration of its role in drought stress tolerance".GENE 665(2018):41-48.
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