De novo transcriptome sequencing of Camellia sasanqua and the analysis of major candidate genes related to floral traits

doi:10.1016/j.plaphy.2017.08.028

	De novo transcriptome sequencing of Camellia sasanqua and the analysis of major candidate genes related to floral traits
	Huang, Hui 1; Xia, En-Hua1 ; Zhang, Hai-Bin 1; Yao, Qiu-Yang 1; Gao, Li-Zhi1
	2017
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	120 页码:103-111
摘要	Camellia sasanqua is one of the most famous horticultural plants in Camellia (Theaceae) due to its aesthetic appeal as landscape plant. Knowledge regarding the genetic basis of flowering time, floral aroma and color in C. sasanqua is limited, but is essential to breed new varieties with desired floral traits. Here, we described the de novo transcriptome of young leaves, flower buds and flowers of C. sasanqua. A total of 60,127 unigenes were functionally annotated based on the sequence similarity. After analysis, we found that two floral integrator genes, SOC1 and AP1, in flowering time pathway showed evidence of gene family expansion. Compared with 1-deoxy-D-xylulose-5-phosphate pathway, some genes in the mevalonate pathway were most highly expressed, suggesting that this might represent the major pathway for terpenoid biosynthesis related to floral aroma in C sasanqua. In flavonoid biosynthesis pathway, PAL, CHI, DFR and ANS showing significantly higher expression levels in flowers and flower buds might have important role in regulation of floral color. The top five most transcription factors (TFs) families in C. sasanqua transcriptome were MYB, MIKC, C3H, FAR1 and HD-ZIP, many of which have a direct relationship with floral traits. In addition, we also identified 33,540 simple sequence repeats (SSRs) in the C sasanqua transcriptome. Collectively, the C sasanqua transcriptome dataset generated from this study along with the SSR markers provide a new resource for the identification of novel regulatory transcripts and will accelerate the genetic improvement of C sasanqua breeding programs. (C) 2017 Published by Elsevier Masson SAS.
关键词	Camellia Sasanqua Floral Traits Signal Pathways Simple Sequence Repeats Transcriptome Sequencing
DOI	10.1016/j.plaphy.2017.08.028
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/60631
专题	中国西南野生生物种质资源库
作者单位	1.[Huang, Hui 2.Xia, En-Hua 3.Zhang, Hai-Bin 4.Yao, Qiu-Yang 5.Gao, Li-Zhi] Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China 6.[Gao, Li-Zhi] South China Agr Univ, Inst Genom & Bioinformat, Guangzhou 510642, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Huang, Hui,Xia, En-Hua,Zhang, Hai-Bin,et al. De novo transcriptome sequencing of Camellia sasanqua and the analysis of major candidate genes related to floral traits[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2017,120:103-111.
APA	Huang, Hui,Xia, En-Hua,Zhang, Hai-Bin,Yao, Qiu-Yang,&Gao, Li-Zhi.(2017).De novo transcriptome sequencing of Camellia sasanqua and the analysis of major candidate genes related to floral traits.PLANT PHYSIOLOGY AND BIOCHEMISTRY,120,103-111.
MLA	Huang, Hui,et al."De novo transcriptome sequencing of Camellia sasanqua and the analysis of major candidate genes related to floral traits".PLANT PHYSIOLOGY AND BIOCHEMISTRY 120(2017):103-111.

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