Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum
Kinoshita, Akihiko1; Nara, Kazuhide2; Sasaki, Hiromi3; Feng, Bang4; Obase, Keisuke5; Yang, Zhu L.4; Yamanaka, Takashi5
2018-03-28
Source PublicationPLOS ONE
ISSN1932-6203
Volume13Issue:3Pages:e0193745
AbstractBlack truffles that morphologically resemble Tuber indicum have been known to occur in Japan since 1979. Our previous studies showed that there are two phylotypes of these truffles, both of which fell into the T. indicum complex (hereinafter "Tubersp. 6" and "Tuber sp. 7"). However, their taxonomic treatment is still unclear. In this study, we conducted morphological and phylogenetic analyses for a total of 52 specimens from Japan (16 Tuber sp. 6 and 13 Tubersp. 7), China (10 T. himalayense and 8 T. indicum), and Taiwan (5 T. formosanum). We compared ascospore ornamentation, size, distribution of asci with average number of spores per ascus, spine size and shape of the Japanese specimens with their allied taxa. For phylogenetic analysis, we sequenced two mating loci (MAT1-1-1 and MAT-12-1) and three commonly used loci (ITS, beta-tubulin, and TEF1-alpha). Three distinct lineages were recognized by phylogenetic analyses based on the sequences of the two mating related loci and three independent loci. The Tubersp. 6 sequences clustered with those of T. himalayense and T. formosanum, and there was no clear difference in morphology among them. Tubersp. 7 formed a distinct lineage in each phylogram. The specimens tended to have five-spored asci more frequently than other allied species and could be characterized as having ascospore ornamentation with longer spines and narrower spine bases. We therefore described Tubersp. 7 as a new species (T. longispinosum), and treat Tuber sp. 6 and T. formosanum as synonyms of T. himalayense.
DOI10.1371/journal.pone.0193745
Language英语
WOS IDWOS:000428603100029
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/60434
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Forestry & Forest Prod Res Inst, Kyushu Res Ctr, Kumamoto, Kumamoto, Japan
2.Univ Tokyo, Grad Sch Frontier Sci, Kashiwa, Chiba, Japan
3.Mycologist Circle Japan, Fujisawa, Kanagawa, Japan
4.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China
5.Forestry & Forest Prod Res Inst, Dept Mushroom Sci & Forest Microbiol, Tsukuba, Ibaraki, Japan
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GB/T 7714
Kinoshita, Akihiko,Nara, Kazuhide,Sasaki, Hiromi,et al. Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum[J]. PLOS ONE,2018,13(3):e0193745.
APA Kinoshita, Akihiko.,Nara, Kazuhide.,Sasaki, Hiromi.,Feng, Bang.,Obase, Keisuke.,...&Yamanaka, Takashi.(2018).Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum.PLOS ONE,13(3),e0193745.
MLA Kinoshita, Akihiko,et al."Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum".PLOS ONE 13.3(2018):e0193745.
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