Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes

doi:10.1038/s41598-017-16102-0

	Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes
	Wang, Qiuli 1,2,3,4; Yin, Xin 1,2,3,5; Chen, Qian 1,2,3; Xiang, Nan 1,2,3; Sun, Xudong 1,2,3; Yang, Yunqiang1,2,3 ; Yang, Yongping1,2,3
	2017-11-17
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	7 页码:15803
摘要	Calcium-dependent protein kinases (CDPKs) as crucial sensors of calcium concentration changes play important roles in responding to abiotic and biotic stresses. In this study, 55 BrrCDPK genes, which were phylogenetically clustered into four subfamilies, were identified. Chromosome locations indicated that the CDPK family in turnip expanded by segmental duplication and genome rearrangement. Moreover, gene expression profiles showed that different BrrCDPKs were expressed in specific tissues or stages. Transcript levels of BrrCDPKs indicated that they were involved in abiotic and biotic stresses and that paralogs exhibited functional divergence. Additionally, we identified 15 Rboh genes in turnip; the results of yeast two-hybrid analysis suggested that BrrRbohD1 interacted only with BrrCDPK10 and that BrrRbohD2 interacted with BrrCDPK4/7/9/10/17/22/23. Most of the genes play an important role in pst DC3000 defense by regulating the accumulation of H2O2 and stomatal closure. Our study may provide an important foundation for future functional analysis of BrrCDPKs and reveal further biological roles.
DOI	10.1038/s41598-017-16102-0
语种	英语
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/60349
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China 4.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qiuli,Yin, Xin,Chen, Qian,et al. Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes[J]. SCIENTIFIC REPORTS,2017,7:15803.
APA	Wang, Qiuli.,Yin, Xin.,Chen, Qian.,Xiang, Nan.,Sun, Xudong.,...&Yang, Yongping.(2017).Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes.SCIENTIFIC REPORTS,7,15803.
MLA	Wang, Qiuli,et al."Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes".SCIENTIFIC REPORTS 7(2017):15803.

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