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ISSR DIVERSITY AND GENETIC DIFFERENTIATION OF ANCIENT TEA (CAMELLIA SINENSIS VAR. ASSAMICA) PLANTATIONS FROM CHINA: IMPLICATIONS FOR PRECIOUS TEA GERMPLASM CONSERVATION
Ji, P. Z.1,2; Li, H.1; Gao, L. Z.3; Zhang, J.2; Cheng, Z. Q.4; Huang, X. Q.1
通讯作者Huang, XQ (reprint author), Yunnan Univ, Coll Life Sci, Kunming 650091, Peoples R China
2011-02-01
发表期刊PAKISTAN JOURNAL OF BOTANY
ISSN0556-3321
卷号43期号:1页码:281-291
摘要Over 10 centuries, ancient cultivated tea populations (Camellia sinensis var. assamica) are still planted merely in Yunnan province of China. Genetic diversity and differentiation were examined in 10 ancient tea plantations by using ISSR markers. The average genetic diversity within populations, estimated with Nei's genetic diversity (H(E)), was approximately 0.2809, while Shannon indices (H(O)) was 0.4179. The percentage of polymorphic loci (P) of the 10 populations ranged from 56.5% to 90.91%. We found a moderate level of genetic differentiation among population as evidenced by the coefficients of gene differentiation (Gst) of 0.3911 and the analysis of molecular variance (AMOVA) of 39.70%. The result could be explained by the nature of highly out crossing in the tea species as well as serious habitat fragmentation. Finally, conservation strategies were discussed to protect these ancient tea populations, including in situ reserve settings and ex situ germplasm sampling.
关键词Polymerase-chain-reaction Rapd Markers Cultivated Tea Populations Amplification Rare Dna Classification Distances Clones
学科领域Plant Sciences
收录类别SCI
语种英语
WOS记录号WOS:000288090700029
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/5201
专题中国西南野生生物种质资源库
作者单位1.Yunnan Univ, Coll Life Sci, Kunming 650091, Peoples R China
2.Yunnan Acad Agr Sci, Tea Res Inst, Menghai 666201, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
4.Yunnan Acad Agr Sci, Biotechnol & Genet Germplasm Inst, Kunming 650223, Peoples R China
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Ji, P. Z.,Li, H.,Gao, L. Z.,et al. ISSR DIVERSITY AND GENETIC DIFFERENTIATION OF ANCIENT TEA (CAMELLIA SINENSIS VAR. ASSAMICA) PLANTATIONS FROM CHINA: IMPLICATIONS FOR PRECIOUS TEA GERMPLASM CONSERVATION[J]. PAKISTAN JOURNAL OF BOTANY,2011,43(1):281-291.
APA Ji, P. Z.,Li, H.,Gao, L. Z.,Zhang, J.,Cheng, Z. Q.,&Huang, X. Q..(2011).ISSR DIVERSITY AND GENETIC DIFFERENTIATION OF ANCIENT TEA (CAMELLIA SINENSIS VAR. ASSAMICA) PLANTATIONS FROM CHINA: IMPLICATIONS FOR PRECIOUS TEA GERMPLASM CONSERVATION.PAKISTAN JOURNAL OF BOTANY,43(1),281-291.
MLA Ji, P. Z.,et al."ISSR DIVERSITY AND GENETIC DIFFERENTIATION OF ANCIENT TEA (CAMELLIA SINENSIS VAR. ASSAMICA) PLANTATIONS FROM CHINA: IMPLICATIONS FOR PRECIOUS TEA GERMPLASM CONSERVATION".PAKISTAN JOURNAL OF BOTANY 43.1(2011):281-291.
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