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题名: Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
作者: Li, Wei1, 2, 3; Xiang, Fen1; Zhong, Micai2; Zhou, Lingyun1; Liu, Hongyan1; Li, Saijun1; Wang, Xuewen2, 4
刊名: SCIENTIFIC REPORTS
英文摘要: Applied nitrogen (N) fertilizer significantly increases the leaf yield. However, most N is not utilized by the plant, negatively impacting the environment. To date, little is known regarding N utilization genes and mechanisms in the leaf production. To understand this, we investigated transcriptomes using RNA-seq and amino acid levels with N treatment in tea (Camellia sinensis), the most popular beverage crop. We identified 196 and 29 common differentially expressed genes in roots and leaves, respectively, in response to ammonium in two tea varieties. Among those genes, AMT, NRT and AQP for N uptake and GOGAT and GS for N assimilation were the key genes, validated by RT-qPCR, which expressed in a network manner with tissue specificity. Importantly, only AQP and three novel DEGs associated with stress, manganese binding, and gibberellin-regulated transcription factor were common in N responses across all tissues and varieties. A hypothesized gene regulatory network for N was proposed. A strong statistical correlation between key genes' expression and amino acid content was revealed. The key genes and regulatory network improve our understanding of the molecular mechanism of N usage and offer gene targets for plant improvement.
出版日期: 2017-05-10
卷号: 7, 页码:1693
DOI标识: 10.1038/s41598-017-01949-0
语种: 英语
收录类别: SCI
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/51244
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Hunan Acad Agr Sci, Tea Res Inst, Natl Ctr Tea Improvement, Hunan Branch, 702 Yuanda Rd, Changsha 410125, Hunan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, 132 Lanhei Rd, Kunming 650201, Peoples R China
3.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China
4.Dept Genet, 120 E Green St, Athens, GA 30602 USA

Recommended Citation:
Li, Wei,Xiang, Fen,Zhong, Micai,et al. Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)[J]. SCIENTIFIC REPORTS,2017,7:1693.
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