Molecular cloning and functional analysis of a novel phytoglobin gene from the alpine plant Stipa purpurea
Li, Xiong1,2; Sun, Xudong1,2; Yang, Shihai3; Qian, Min1,2; Yang, Yunqiang1,2; Yang, Yongping1,2
2017
Source PublicationPLANT ECOLOGY & DIVERSITY
Volume10Issue:1Pages:17-27
AbstractBackground: Stipa purpurea, the dominant species of the alpine steppe in the Tibetan Plateau, survives well under various abiotic stresses including drought, hypoxia and chilling.Aims: To understand the molecular mechanism by which alpine plants including S. purpurea adapt to the abiotic environmental factors of the Tibetan Plateau.Methods: We cloned the coding domain sequence of one Phytogb gene from S. purpurea using the rapid amplification of cDNA ends method and analysed its function by heterologous expression in Arabidopsis.Results: The Phytogb gene, which we named StipurPhytogb1, included 474 nucleotides and encoded a protein of 157 amino acids. Functional analyses showed that StipurPhytogb1 could improve the resistance of transgenic Arabidopsis to drought and hypoxia caused by waterlogging. Further analyses suggested that StipurPhytogb1 improved the above resistance by inhibiting nitric oxide accumulation and mediating the induction of antioxidant enzyme activities to scavenge reactive oxygen species.Conclusion: These findings indicate that StipurPhytogb1 may play an essential role in the adaptation of S. purpurea to drought and the hypoxic environment of the Tibetan Plateau and has potential as a candidate gene for genetic transformation to improve plant stress tolerance.
KeywordEnvironmental Adaptation Heterologous Expression Phytoglobin Stipa Purpurea Tibetan Plateau
DOI10.1080/17550874.2017.1302998
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000401516900003
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/51227
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing, Peoples R China
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Li, Xiong,Sun, Xudong,Yang, Shihai,et al. Molecular cloning and functional analysis of a novel phytoglobin gene from the alpine plant Stipa purpurea[J]. PLANT ECOLOGY & DIVERSITY,2017,10(1):17-27.
APA Li, Xiong,Sun, Xudong,Yang, Shihai,Qian, Min,Yang, Yunqiang,&Yang, Yongping.(2017).Molecular cloning and functional analysis of a novel phytoglobin gene from the alpine plant Stipa purpurea.PLANT ECOLOGY & DIVERSITY,10(1),17-27.
MLA Li, Xiong,et al."Molecular cloning and functional analysis of a novel phytoglobin gene from the alpine plant Stipa purpurea".PLANT ECOLOGY & DIVERSITY 10.1(2017):17-27.
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