KIB OpenIR  > 中国西南野生生物种质资源库
Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis
Ling, Li-Zhen1,2; Zhang, Shu-Dong3; Zhao, Fan1; Yang, Jin-Long1; Song, Wen-Hui1; Guan, Shen-Min1; Li, Xin-Shu1; Huang, Zhuang-Jia1; Cheng, Le1,2,4
2017
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume18Issue:1Pages:219
AbstractLong dormancy period of seeds limits the large-scale artificial cultivation of the scarce Paris polyphylla var. yunnanensis, an important traditional Chinese medicine. Characterizing miRNAs and their targets is crucial to understanding the role of miRNAs during seed dormancy in this species. Considering the limited genome information of this species, we first sequenced and assembled the transcriptome data of dormant seeds and their seed coats as the reference genome. A total of 146,671 unigenes with an average length of 923 bp were identified and showed functional diversity based on different annotation methods. Two small RNA libraries from respective seeds and seed coats were sequenced and the combining data indicates that 263 conserved miRNAs belonging to at least 83 families and 768 novel miRNAs in 1174 transcripts were found. The annotations of the predicted putative targets of miRNAs suggest that these miRNAs were mainly involved in the cell, metabolism and genetic information processing by direct and indirect regulation patterns in dormant seeds of P. polyphylla var. yunnanensis. Therefore, we provide the first known miRNA profiles and their targets, which will assist with further study of the molecular mechanism of seed dormancy in P. polyphylla var. yunnanensis.
KeywordParis Polyphylla Var. Yunnanensis Mirnas Seed Dormancy High-throughput Sequencing Technology
DOI10.3390/ijms18010219
Indexed BySCI
Language英语
WOS IDWOS:000393030600213
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/51009
Collection中国西南野生生物种质资源库
Affiliation1.BGI Yunnan, Kunming 650106, Peoples R China
2.BGI Shenzhen, China Natl GeneBank, Shenzhen 518083, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
4.Dali Univ, Coll Clin Med, Coll Basic Med Sci, Dali 671000, Peoples R China
Recommended Citation
GB/T 7714
Ling, Li-Zhen,Zhang, Shu-Dong,Zhao, Fan,et al. Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(1):219.
APA Ling, Li-Zhen.,Zhang, Shu-Dong.,Zhao, Fan.,Yang, Jin-Long.,Song, Wen-Hui.,...&Cheng, Le.(2017).Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(1),219.
MLA Ling, Li-Zhen,et al."Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.1(2017):219.
Files in This Item: Download All
File Name/Size DocType Version Access License
ijms-18-00219.pdf(891KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ling, Li-Zhen]'s Articles
[Zhang, Shu-Dong]'s Articles
[Zhao, Fan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ling, Li-Zhen]'s Articles
[Zhang, Shu-Dong]'s Articles
[Zhao, Fan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ling, Li-Zhen]'s Articles
[Zhang, Shu-Dong]'s Articles
[Zhao, Fan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: ijms-18-00219.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.