| Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis | |
| Ling, Li-Zhen1,2; Zhang, Shu-Dong3; Zhao, Fan1; Yang, Jin-Long1; Song, Wen-Hui1; Guan, Shen-Min1; Li, Xin-Shu1; Huang, Zhuang-Jia1; Cheng, Le1,2,4 | |
| 2017 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 卷号 | 18期号:1页码:219 |
| 摘要 | Long dormancy period of seeds limits the large-scale artificial cultivation of the scarce Paris polyphylla var. yunnanensis, an important traditional Chinese medicine. Characterizing miRNAs and their targets is crucial to understanding the role of miRNAs during seed dormancy in this species. Considering the limited genome information of this species, we first sequenced and assembled the transcriptome data of dormant seeds and their seed coats as the reference genome. A total of 146,671 unigenes with an average length of 923 bp were identified and showed functional diversity based on different annotation methods. Two small RNA libraries from respective seeds and seed coats were sequenced and the combining data indicates that 263 conserved miRNAs belonging to at least 83 families and 768 novel miRNAs in 1174 transcripts were found. The annotations of the predicted putative targets of miRNAs suggest that these miRNAs were mainly involved in the cell, metabolism and genetic information processing by direct and indirect regulation patterns in dormant seeds of P. polyphylla var. yunnanensis. Therefore, we provide the first known miRNA profiles and their targets, which will assist with further study of the molecular mechanism of seed dormancy in P. polyphylla var. yunnanensis. |
| 关键词 | Paris Polyphylla Var. Yunnanensis Mirnas Seed Dormancy High-throughput Sequencing Technology |
| DOI | 10.3390/ijms18010219 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000393030600213 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/51009 |
| 专题 | 中国西南野生生物种质资源库 |
| 作者单位 | 1.BGI Yunnan, Kunming 650106, Peoples R China 2.BGI Shenzhen, China Natl GeneBank, Shenzhen 518083, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China 4.Dali Univ, Coll Clin Med, Coll Basic Med Sci, Dali 671000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, Li-Zhen,Zhang, Shu-Dong,Zhao, Fan,et al. Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(1):219. |
| APA | Ling, Li-Zhen.,Zhang, Shu-Dong.,Zhao, Fan.,Yang, Jin-Long.,Song, Wen-Hui.,...&Cheng, Le.(2017).Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(1),219. |
| MLA | Ling, Li-Zhen,et al."Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.1(2017):219. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| ijms-18-00219.pdf（891KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论