DNA barcoding of Pedicularis L. (Orobanchaceae): Evaluating four universal barcode loci in a large and hemiparasitic genus

doi:10.1111/j.1759-6831.2011.00154.x

	DNA barcoding of Pedicularis L. (Orobanchaceae): Evaluating four universal barcode loci in a large and hemiparasitic genus
	Yu, Wen-Bin 1,2,3; Huang, Pan-Hui 1,2,3; Ree, Richard H.4; Liu, Min-Lu1,2,3 ; Li, De-Zhu1,2 ; Wang, Hong1,2
通讯作者	wanghong@mail.kib.ac.cn
	2011-09-01
发表期刊	JOURNAL OF SYSTEMATICS AND EVOLUTION
ISSN	1674-4918
卷号	49 期号:5 页码:425-437
摘要	One application of DNA barcoding is species identification based on sequences of a short and standardized DNA region. In plants, various DNA regions, alone or in combination, have been proposed and investigated, but consensus on a universal plant barcode remains elusive. In this study, we tested the utility of four candidate barcoding regions (rbcL, matK, trnH-psbA, and internal transcribed spacer (ITS)) as DNA barcodes for discriminating species in a large and hemiparasitic genus Pedicularis (Orobanchaceae). Amplification and sequencing was successful using single primer pairs for rbcL, trnH-psbA, and ITS, whereas two primer pairs were required for matK. Patterns of sequence divergence commonly showed a "barcoding gap", that is, a bimodal frequency distribution of pairwise distances representing genetic diversity within and between species, respectively. Considering primer universality, ease of amplification and sequencing, and performance in discriminating species, we found the most effective single-region barcode for Pedicularis to be ITS, and the most effective two-region barcode to be rbcL + ITS. Both discriminated at least 78% of the 88 species and correctly identified at least 89% of the sequences in our sample, and were effective in placing unidentified samples in known species groups. Our results suggest that DNA barcoding has the potential to aid taxonomic research in Pedicularis, a species-rich cosmopolitan clade much in need of revision, as well as ecological studies in its center of diversity, the Hengduan Mountains region of China.
关键词	Dna Barcode Hengduan Mountains Region Its Pedicularis Rbcl Species Identification
学科领域	Plant Sciences
DOI	10.1111/j.1759-6831.2011.00154.x
收录类别	SCI
语种	英语
WOS记录号	WOS:000295322200006
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/3808
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Kunming Inst Bot, Kunming 650204, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Field Museum Nat Hist, Dept Bot, Chicago, IL 60605 USA
推荐引用方式 GB/T 7714	Yu, Wen-Bin,Huang, Pan-Hui,Ree, Richard H.,et al. DNA barcoding of Pedicularis L. (Orobanchaceae): Evaluating four universal barcode loci in a large and hemiparasitic genus[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(5):425-437.
APA	Yu, Wen-Bin,Huang, Pan-Hui,Ree, Richard H.,Liu, Min-Lu,Li, De-Zhu,&Wang, Hong.(2011).DNA barcoding of Pedicularis L. (Orobanchaceae): Evaluating four universal barcode loci in a large and hemiparasitic genus.JOURNAL OF SYSTEMATICS AND EVOLUTION,49(5),425-437.
MLA	Yu, Wen-Bin,et al."DNA barcoding of Pedicularis L. (Orobanchaceae): Evaluating four universal barcode loci in a large and hemiparasitic genus".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.5(2011):425-437.

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