Genetic diversity of Meconopsis integrifolia (Maxim.) Franch. In the East Himalaya-Hengduan Mountains inferred from fluorescent amplified fragment length polymorphism analysis

doi:10.1016/j.bse.2016.08.007

	Genetic diversity of Meconopsis integrifolia (Maxim.) Franch. In the East Himalaya-Hengduan Mountains inferred from fluorescent amplified fragment length polymorphism analysis
	Guo, Jian-Ling 1; Zhang, Xiao-Yun 1; Zhang, Jian-Wen2 ; Li, Zhi-Min 1,3; Sun, Wen-Guang1,2 ; Zhang, Yong-Hong 1
	2016-12-01
发表期刊	BIOCHEMICAL SYSTEMATICS AND ECOLOGY
卷号	69 页码:67-75
摘要	Meconopsis integrifolia (Maxim.) Franch. is a representative species of the genus found in the East Himalaya-Hengduan Mountain. As a species of medical and horticultural significance, M. integrifolia is threatened by over-exploitation and habitat fragmentation. In this study, the genetic diversity and structure of M. integrifolia, represented by 183 individuals from 10 populations, were studied using 6 pairs of fluorescent Amplified Fragment Length Polymorphisms primers. Our results showed a relatively high genetic variation at the species level (PPB = 82.0%, H-E = 0.2356 and I = 0.3695). In contrast, population diversity was extremely low, as measured by Nei's genetic diversity index and Shannon's diversity index (H-E = 0.0317, 1 = 0.0480). Analyses of molecular variance (AMOVA) showed that among and within populations, the genetic variation accounted for 88.9% and 11.1% of the total genetic variation, respectively. In addition, Nei's coefficient of differentiation (G(ST)) was found to be high (0.8636), confirming the significant high level of genetic differentiation as well as low gene flow among populations of this species. The results of Neighbor joining cluster, PCoA, and Bayesian assignment revealed similar genetic differentiation patterns, suggesting three main genetic groups that are concordant with their geographical distribution. Three main barriers-the Mekong River, the Yalong River, and a branch of the Yalong River-were detected using BARRIER software. The possible mechanisms and implications of these findings for conservation are discussed. (C) 2016 Elsevier Ltd. All rights reserved.
关键词	Meconopsis Integrifolia Aflp Genetic Diversity Barrier East Himalaya-hengduan Mountains
DOI	10.1016/j.bse.2016.08.007
收录类别	SCI
语种	英语
WOS记录号	WOS:000390497300010
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/33792
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Yunnan Normal Univ, Dept Life Sci, Kunming 650500, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China 3.Minist Educ, Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Kunming 650500, Peoples R China
推荐引用方式 GB/T 7714	Guo, Jian-Ling,Zhang, Xiao-Yun,Zhang, Jian-Wen,et al. Genetic diversity of Meconopsis integrifolia (Maxim.) Franch. In the East Himalaya-Hengduan Mountains inferred from fluorescent amplified fragment length polymorphism analysis[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2016,69:67-75.
APA	Guo, Jian-Ling,Zhang, Xiao-Yun,Zhang, Jian-Wen,Li, Zhi-Min,Sun, Wen-Guang,&Zhang, Yong-Hong.(2016).Genetic diversity of Meconopsis integrifolia (Maxim.) Franch. In the East Himalaya-Hengduan Mountains inferred from fluorescent amplified fragment length polymorphism analysis.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,69,67-75.
MLA	Guo, Jian-Ling,et al."Genetic diversity of Meconopsis integrifolia (Maxim.) Franch. In the East Himalaya-Hengduan Mountains inferred from fluorescent amplified fragment length polymorphism analysis".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 69(2016):67-75.

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