| Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants | |
Yang, Guo-Qian1,2 ; Chen, Yun-Mei1,2; Wang, Jin-Peng3; Guo, Cen1,2; Zhao, Lei1,2; Wang, Xiao-Yan1; Guo, Ying1,2; Li, Li3; Li, De-Zhu1; Guo, Zhen-Hua1
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| 2016-08-04 | |
| 发表期刊 | PLANT METHODS
 |
| 卷号 | 12期号:1页码:39 |
| 摘要 | Background: The double digest restriction-site associated DNA sequencing technology (ddRAD-seq) is a reduced representation sequencing technology by sampling genome-wide enzyme loci developed on the basis of next-generation sequencing. ddRAD-seq has been widely applied to SNP marker development and genotyping on animals, especially on marine animals as the original ddRAD protocol is mainly built and trained based on animal data. However, wide application of ddRAD-seq technology in plant species has not been achieved so far. Here, we aim to develop an optimized ddRAD library preparation protocol be accessible to most angiosperm plant species without much startup pre-experiment and costs. |
| 关键词 | Rad-seq Ddrad Middrad Genotype-by-sequencing Next-generation Sequencing |
| DOI | 10.1186/s13007-016-0139-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000382353900001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/33602 |
| 专题 | 中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China 2.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming 650201, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Guo-Qian,Chen, Yun-Mei,Wang, Jin-Peng,et al. Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants[J]. PLANT METHODS,2016,12(1):39. |
| APA | Yang, Guo-Qian.,Chen, Yun-Mei.,Wang, Jin-Peng.,Guo, Cen.,Zhao, Lei.,...&Guo, Zhen-Hua.(2016).Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants.PLANT METHODS,12(1),39. |
| MLA | Yang, Guo-Qian,et al."Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants".PLANT METHODS 12.1(2016):39. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 2016_YangGQ_Pl_Metho（3378KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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