| Telling plant species apart with DNA: from barcodes to genomes | |
Hollingsworth, Peter M.; Li, De-Zhu ; van der Bank, Michelle; Twyford, Alex D.
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| 2016-09-05 | |
| 发表期刊 | PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
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| 卷号 | 371期号:1702 |
| 摘要 | Land plants underpin a multitude of ecosystem functions, support human livelihoods and represent a critically important component of terrestrial biodiversity-yet many tens of thousands of species await discovery, and plant identification remains a substantial challenge, especially where material is juvenile, fragmented or processed. In this opinion article, we tackle two main topics. Firstly, we provide a short summary of the strengths and limitations of plant DNA barcoding for addressing these issues. Secondly, we discuss options for enhancing current plant barcodes, focusing on increasing discriminatory power via either gene capture of nuclear markers or genome skimming. The former has the advantage of establishing a defined set of target loci maximizing efficiency of sequencing effort, data storage and analysis. The challenge is developing a probe set for large numbers of nuclear markers that works over sufficient phylogenetic breadth. Genome skimming has the advantage of using existing protocols and being backward compatible with existing barcodes; and the depth of sequence coverage can be increased as sequencing costs fall. Its non-targeted nature does, however, present a major informatics challenge for upscaling to large sample sets. |
| 关键词 | Plant Dna Barcoding Next-generation Sequencing Genome Skimming Species Discrimination Hybrid Baits |
| DOI | 10.1098/rstb.2015.0338 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000381779700007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/28598 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 推荐引用方式 GB/T 7714 | Hollingsworth, Peter M.,Li, De-Zhu,van der Bank, Michelle,et al. Telling plant species apart with DNA: from barcodes to genomes[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,2016,371(1702). |
| APA | Hollingsworth, Peter M.,Li, De-Zhu,van der Bank, Michelle,&Twyford, Alex D..(2016).Telling plant species apart with DNA: from barcodes to genomes.PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,371(1702). |
| MLA | Hollingsworth, Peter M.,et al."Telling plant species apart with DNA: from barcodes to genomes".PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 371.1702(2016). |
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| 20150338.full.pdf（904KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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