Insights into the Genetic Relationships and Breeding Patterns of the African Tea Germplasm Based on nSSR Markers and cpDNA Sequences

doi:10.3389/fpls.2076.07244

	Insights into the Genetic Relationships and Breeding Patterns of the African Tea Germplasm Based on nSSR Markers and cpDNA Sequences
	Wambulwa, Moses C.; Meegahakumbura, Muditha K.; Kamunya, Samson; Muchugi, Alice; Moller, Michael; Liu, Jie; Xu, Jian-Chu; Ranjitkar, Sailesh; Li, De-Zhu; Gao, Lian-Ming
	2016-08-30
发表期刊	FRONTIERS IN PLANT SCIENCE
卷号	7
摘要	Africa is one of the key centers of global tea production. Understanding the genetic diversity and relationships of cultivars of African tea is important for future targeted breeding efforts for new crop cultivars, specialty tea processing, and to guide germplasm conservation efforts. Despite the economic importance of tea in Africa, no research work has been done so far on its genetic diversity at a continental scale. Twenty-three nSSRs and three plastid DNA regions were used to investigate the genetic diversity, relationships, and breeding patterns of tea accessions collected from eight countries of Africa. A total of 280 African tea accessions generated 297 alleles with a mean of 12.91 alleles per locus and a genetic diversity (H-S) estimate of 0.652. A STRUCTURE analysis suggested two main genetic groups of African tea accessions which corresponded well with the two tea types Camellia sinensis var. sinensis and C. sinensis var. assamica, respectively, as well as an admixed "mosaic" group whose individuals were defined as hybrids of F2 and BC generation with a high proportion of C. sinensis var. assamica being maternal parents. Accessions known to be C. sinensis var. assamica further separated into two groups representing the two major tea breeding centers corresponding to southern Africa (Tea Research Foundation of Central Africa, TRFCA), and East Africa (Tea Research Foundation of Kenya, TRFK). Tea accessions were shared among countries. African tea has relatively lower genetic diversity. C. sinensis var. assamica is the main tea type under cultivation and contributes more in tea breeding improvements in Africa. International germplasm exchange and movement among countries within Africa was confirmed. The clustering into two main breeding centers, TRFCA, and TRFK, suggested that some traits of C. sinensis var. assamica and their associated genes possibly underwent selection during geographic differentiation or local breeding preferences. This study represents the first step toward effective utilization of differently inherited molecular markers for exploring the breeding status of African tea. The findings here will be important for planning the exploration, utilization, and conservation of tea germplasm for future breeding efforts in Africa.
关键词	African Tea Germplasm Breeding Improvement Camellia Sinensis Genetic Diversity Nssr Markers Cpdna Regions
DOI	10.3389/fpls.2076.07244
收录类别	SCI
语种	英语
WOS记录号	WOS:000382121400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/28586
专题	中国科学院东亚植物多样性与生物地理学重点实验室
推荐引用方式 GB/T 7714	Wambulwa, Moses C.,Meegahakumbura, Muditha K.,Kamunya, Samson,et al. Insights into the Genetic Relationships and Breeding Patterns of the African Tea Germplasm Based on nSSR Markers and cpDNA Sequences[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Wambulwa, Moses C..,Meegahakumbura, Muditha K..,Kamunya, Samson.,Muchugi, Alice.,Moller, Michael.,...&Gao, Lian-Ming.(2016).Insights into the Genetic Relationships and Breeding Patterns of the African Tea Germplasm Based on nSSR Markers and cpDNA Sequences.FRONTIERS IN PLANT SCIENCE,7.
MLA	Wambulwa, Moses C.,et al."Insights into the Genetic Relationships and Breeding Patterns of the African Tea Germplasm Based on nSSR Markers and cpDNA Sequences".FRONTIERS IN PLANT SCIENCE 7(2016).

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