KIB OpenIR  > 中国西南野生生物种质资源库
Genomic DNA Methylation Analyses Reveal the Distinct Profiles in Castor Bean Seeds with Persistent Endosperms
Xu, Wei; Yang, Tianquan; Dong, Xue; Li, De-Zhu; Liu, Aizhong
2016-06-01
Source PublicationPLANT PHYSIOLOGY
Volume171Issue:2Pages:1242-1258
AbstractInvestigations of genomic DNA methylation in seeds have been restricted to a few model plants. The endosperm genomic DNA hypomethylation has been identified in angiosperm, but it is difficult to dissect the mechanism of how this hypomethylation is established and maintained because endosperm is ephemeral and disappears with seed development in most dicots. Castor bean (Ricinus communis), unlike Arabidopsis (Arabidopsis thaliana), endosperm is persistent throughout seed development, providing an excellent model in which to dissect the mechanism of endosperm genomic hypomethylation in dicots. We characterized the DNA methylation-related genes encoding DNA methyltransferases and demethylases and analyzed their expression profiles in different tissues. We examined genomic methylation including CG, CHG, and CHH contexts in endosperm and embryo tissues using bisulfite sequencing and revealed that the CHH methylation extent in endosperm and embryo was, unexpectedly, substantially higher than in previously studied plants, irrespective of the CHH percentage in their genomes. In particular, we found that the endosperm exhibited a global reduction in CG and CHG methylation extents relative to the embryo, markedly switching global gene expression. However, CHH methylation occurring in endosperm did not exhibit a significant reduction. Combining with the expression of 24-nucleotide small interfering RNAs (siRNAs) mapped within transposable element (TE) regions and genes involved in the RNA-directed DNA methylation pathway, we demonstrate that the 24-nucleotide siRNAs played a critical role in maintaining CHH methylation and repressing the activation of TEs in persistent endosperm development. This study discovered a novel genomic DNA methylation pattern and proposes the potential mechanism occurring in dicot seeds with persistent endosperm.
DOI10.1104/pp.16.00056
Indexed BySCI
Language英语
WOS IDWOS:000380699200037
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/28514
Collection中国西南野生生物种质资源库
Recommended Citation
GB/T 7714
Xu, Wei,Yang, Tianquan,Dong, Xue,et al. Genomic DNA Methylation Analyses Reveal the Distinct Profiles in Castor Bean Seeds with Persistent Endosperms[J]. PLANT PHYSIOLOGY,2016,171(2):1242-1258.
APA Xu, Wei,Yang, Tianquan,Dong, Xue,Li, De-Zhu,&Liu, Aizhong.(2016).Genomic DNA Methylation Analyses Reveal the Distinct Profiles in Castor Bean Seeds with Persistent Endosperms.PLANT PHYSIOLOGY,171(2),1242-1258.
MLA Xu, Wei,et al."Genomic DNA Methylation Analyses Reveal the Distinct Profiles in Castor Bean Seeds with Persistent Endosperms".PLANT PHYSIOLOGY 171.2(2016):1242-1258.
Files in This Item: Download All
File Name/Size DocType Version Access License
xu2016.pdf(2909KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Wei]'s Articles
[Yang, Tianquan]'s Articles
[Dong, Xue]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Wei]'s Articles
[Yang, Tianquan]'s Articles
[Dong, Xue]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Wei]'s Articles
[Yang, Tianquan]'s Articles
[Dong, Xue]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: xu2016.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.