Genetic diversity within and among populations of the endangered species Taxus fuana (Taxaceae) from Pakistan and implications for its conservation

doi:10.1016/j.bse.2007.09.012

	Genetic diversity within and among populations of the endangered species Taxus fuana (Taxaceae) from Pakistan and implications for its conservation
	Shah, Amin 1,2; Li, De-Zhu1 ; Gao, Lian-Ming1 ; Li, Hong-Tao1,2 ; Moeller, Michael 3
通讯作者	gaolm@mail.kib.ac.cn
	2008-03-01
发表期刊	BIOCHEMICAL SYSTEMATICS AND ECOLOGY
ISSN	0305-1978
卷号	36 期号:3 页码:183-193
摘要	The West Himalayan yew, Taxus fuana Nan Li & R.R. Mill (Taxaceae), is an endangered species endemic to the Western Himalayas. An investigation of the genetic diversity of wild populations of T.fuana in Pakistan was undertaken. The genetic diversity and genetic structure was quantified using random amplified polymorphic DNA (RAPD) variation in 219 individuals of the 10 populations. Of the 32 universal primers screened 16 produced highly reproducible, clear RAPD bands. Using these primers, 193 discernible DNA fragments were generated, of which 164 (84.97%) were polymorphic. The statistical results indicated that there was a relatively low genetic diversity within populations (with percentages of polymorphic bands, PPB, ranging from 29.53 to 50.26%, with an average of 38.34% and a Nei's genetic diversity index (H-E) of 0.1165), and a high genetic differentiation among populations (G(ST) = 0.5842, Phi(ST) = 0.5685) within these populations. The gene flow (N-m) was low with only 0.3558. The distribution of genetic diversity revealed among the wild populations of T.fuana in Pakistan may result from their evolutionary history including habitat fragmentation, genetic drift combined with limited gene flow. The results are unexpected for an outcrossing, wind-pollinated and long-lived woody species. Based on these findings, conservation concerns are discussed together with possible strategies for implementing in situ and ex situ conservation. (c) 2007 Elsevier Ltd. All rights reserved.
关键词	Gene Flow Genetic Diversity Pakistan Population Structure Rapd Taxus Fuana
学科领域	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI	10.1016/j.bse.2007.09.012
收录类别	SCI
语种	英语
WOS记录号	WOS:000253246900004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2785
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Royal Bot Gardens, Edinburgh EH3 5LR, Midlothian, Scotland
推荐引用方式 GB/T 7714	Shah, Amin,Li, De-Zhu,Gao, Lian-Ming,et al. Genetic diversity within and among populations of the endangered species Taxus fuana (Taxaceae) from Pakistan and implications for its conservation[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2008,36(3):183-193.
APA	Shah, Amin,Li, De-Zhu,Gao, Lian-Ming,Li, Hong-Tao,&Moeller, Michael.(2008).Genetic diversity within and among populations of the endangered species Taxus fuana (Taxaceae) from Pakistan and implications for its conservation.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,36(3),183-193.
MLA	Shah, Amin,et al."Genetic diversity within and among populations of the endangered species Taxus fuana (Taxaceae) from Pakistan and implications for its conservation".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 36.3(2008):183-193.

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