| Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling | |
Zhou, Yanli1,2,3; Sun, Xudong1,2,4; Yang, Yunqiang1,2,4 ; Li, Xiong1,2,4; Cheng, Ying5; Yang, Yongping1,2,3,4
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| 2016-06-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 卷号 | 17期号:6 |
| 摘要 | Stipa purpurea (S. purpurea) is the dominant plant species in the alpine steppe of the Qinghai-Tibet Plateau, China. It is highly resistant to cold and drought conditions. However, the underlying mechanisms regulating the stress tolerance are unknown. In this study, a CIPK gene from S. purpurea (SpCIPK26) was isolated. The SpCIPK26 coding region consisted of 1392 bp that encoded 464 amino acids. The protein has a highly conserved catalytic structure and regulatory domain. The expression of SpCIPK26 was induced by drought and salt stress. SpCIPK26 overexpression in Arabidopsis thaliana (A. thaliana) plants provided increased tolerance to drought and salt stress in an abscisic acid (ABA)-dependent manner. Compared with wild-type A. thaliana plants, SpCIPK26-overexpressing plants had higher survival rates, water potentials, and photosynthetic efficiency (Fv/Fm), as well as lower levels of reactive oxygen species (ROS) following exposure to drought and salt stress. Gene expression analyses indicated stress-inducible genes (RD29A, RD29B, and ABF2) and a ROS-scavenger gene (CAT1) were upregulated in SpCIPK26-overexpressing plants after stress treatments. All of these marker genes are associated with ABA-responsive cis-acting elements. Additionally, the similarities in the gene expression patterns following ABA, mannitol, and NaCl treatments suggest SpCIPK26 has an important role during plant responses to drought and salt stress and in regulating ABA signaling. |
| 关键词 | Cipk Stipa Purpurea Salt Drought Aba Ros |
| DOI | 10.3390/ijms17060966 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378799300178 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/26299 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650201, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res, Kunming 650201, Peoples R China 5.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yanli,Sun, Xudong,Yang, Yunqiang,et al. Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(6). |
| APA | Zhou, Yanli,Sun, Xudong,Yang, Yunqiang,Li, Xiong,Cheng, Ying,&Yang, Yongping.(2016).Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(6). |
| MLA | Zhou, Yanli,et al."Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.6(2016). |
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