Nuclear microsatellites reveal the genetic architecture and breeding history of tea germplasm of East Africa

doi:10.1007/s11295-015-0963-x

	Nuclear microsatellites reveal the genetic architecture and breeding history of tea germplasm of East Africa
	Wambulwa, M. C.1,2,3,4; Meegahakumbura, M. K.1,2,3,5; Chalo, R.6; Kamunya, S.6; Muchugi, A.4; Xu, J. C.7; Liu, J.1 ; Li, D. Z.1,2,3 ; Gao, L. M.1
	2016-02-01
发表期刊	TREE GENETICS & GENOMES
卷号	12 期号:1
摘要	Tea is the most popular non-alcoholic beverage worldwide and is one of the most important tree cash crops in East Africa. However, no comprehensive study has been carried out on the genetic structure and diversity of tea germplasm for this region to date. In the present study, 193 tea accessions held at the ex situ Germplasm Bank of the Tea Research Institute (TRI), Kenya, were analysed using genetic data from 23 polymorphic simple sequence repeat (SSR) loci. A total of 266 alleles were detected with the number of alleles per locus ranging from 4 to 19 with an average of 7.88. Genetic clustering by STRUCTURE was used to correct misidentified accessions based on morphological characters. After reassignment of the tea accessions, Camellia assamica exhibited the lowest genetic diversity (Hs=0.648) despite being the most widely cultivated tea type in the East African region. C. assamica subsp. lasiocalyx showed the highest genetic diversity (Hs=0.76), which supported its origin by hybridization among tea types. Tea cultivars cultivated across the region exhibited lower genetic diversity (Hs=0.661) compared to material held at the ex situ Germplasm Bank of TRI. Tea accessions clustered in the neighbour-joining tree on the basis of geographical origin, pedigree and leaf pigmentation, indicating their common origin. Our results indicated further that East African tea germplasm has a complex breeding history with a majority of the hybrids being F2 generation and backcross plants. C. assamica contributed significantly more genetic materials in the tea breeding programmes in East Africa. This study highlights the importance of ex situ germplasm banks to conserve the highest genetic diversity, which is an important resource for future tea crop improvements in East Africa.
关键词	Camellia Sinensis East Africa Genetic Diversity Nuclear Microsatellites Tea Breeding
DOI	10.1007/s11295-015-0963-x
收录类别	SCI
语种	英语
WOS记录号	WOS:000371329700006
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/25997
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Kunming 650201, Peoples R China 4.World Agroforestry Ctr, Nairobi 30677, Kenya 5.Coconut Res Inst, Lunuwila, Sri Lanka 6.Kenya Agr & Livestock Res Org, Tea Res Inst, Kericho 820, Kenya 7.China & East Asia Node, World Agroforestry Ctr, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Wambulwa, M. C.,Meegahakumbura, M. K.,Chalo, R.,et al. Nuclear microsatellites reveal the genetic architecture and breeding history of tea germplasm of East Africa[J]. TREE GENETICS & GENOMES,2016,12(1).
APA	Wambulwa, M. C..,Meegahakumbura, M. K..,Chalo, R..,Kamunya, S..,Muchugi, A..,...&Gao, L. M..(2016).Nuclear microsatellites reveal the genetic architecture and breeding history of tea germplasm of East Africa.TREE GENETICS & GENOMES,12(1).
MLA	Wambulwa, M. C.,et al."Nuclear microsatellites reveal the genetic architecture and breeding history of tea germplasm of East Africa".TREE GENETICS & GENOMES 12.1(2016).

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