| Molecular Evidence for the Hybrid Origin of Ilex dabieshanensis (Aquifoliaceae) | |
| Shi, Lin1; Li, Naiwei2; Wang, Shuqiong3,4; Zhou, Yubing3,4; Huang, Weijie3,4; Yang, Yuchen3,4; Ma, Yongpeng5; Zhou, Renchao3,4 | |
| 2016-01-25 | |
| 发表期刊 | PLOS ONE
 |
| 卷号 | 11期号:1 |
| 摘要 | Ilex, the largest genus of dioecious woody plants, is a good study system to assess the role of hybridization in speciation and evolution. Ilex dabieshanensis, a tree endemic to Dabieshan Mountains region, was initially described as a new species. Based on morphological intermediacy and sympatric distribution with its putative parental species, I. cornuta and I. latifolia, we proposed it as a natural hybrid between them. In this study, we sequenced one chloroplast intergenic spacer (trnH-psbA) and two nuclear genes (gapC and nepGS) in I. dabieshanensis and its putative parental species to test the hybrid origin hypothesis. Our results showed that there were one to two differentially fixed sequence differences between I. cornuta and I. latifolia at the two nuclear genes. Twelve of the 14 individuals of I. dabieshanensis exhibited additivity in chromatograms on these differentially fixed sites at both nuclear genes, and the remaining two exhibited additivity in chromatograms on the fixed site at only the nepGS gene. Except one haplotype of I. cornuta at the nepGS gene, all of the haplotypes of I. cornuta at the two nuclear genes were well separated from those of I. latifolia, and most haplotypes of I. dabieshanensis were shared with those of I. cornuta and I. latifolia. Phylogenetic analysis of these haplotypes was largely consistent with haplotype network analysis. I. cornuta and I. latifolia differed by two nucleotide substitutions in the chloroplast intergenic spacer, and 12 individuals of I. dabieshanensis had the same sequences as I. latifolia, while the remaining two were identical with I. cornuta. The molecular data provide convincing evidence for the hybrid origin of I. dabieshanensis and asymmetrical direction of hybridization. One haplotype of I. cornuta at the nepGS gene was nested with those of I. latifolia, indicating introgression to I. cornuta. |
| DOI | 10.1371/journal.pone.0147825 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369527800218 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/25977 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Shunde Polytech, Foshan 528333, Peoples R China 2.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing 210014, Jiangsu, Peoples R China 3.Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China 4.Sun Yat Sen Univ, Guangdong Prov Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Lin,Li, Naiwei,Wang, Shuqiong,et al. Molecular Evidence for the Hybrid Origin of Ilex dabieshanensis (Aquifoliaceae)[J]. PLOS ONE,2016,11(1). |
| APA | Shi, Lin.,Li, Naiwei.,Wang, Shuqiong.,Zhou, Yubing.,Huang, Weijie.,...&Zhou, Renchao.(2016).Molecular Evidence for the Hybrid Origin of Ilex dabieshanensis (Aquifoliaceae).PLOS ONE,11(1). |
| MLA | Shi, Lin,et al."Molecular Evidence for the Hybrid Origin of Ilex dabieshanensis (Aquifoliaceae)".PLOS ONE 11.1(2016). |
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