RNA-seq Reveals Complicated Transcriptomic Responses to Drought Stress in a Nonmodel Tropic Plant, Bombax ceiba L.

doi:10.4137/EBO.S20620

	RNA-seq Reveals Complicated Transcriptomic Responses to Drought Stress in a Nonmodel Tropic Plant, Bombax ceiba L.
	Zhou, Zhili; Ma, Huancheng; Lin, Kevin; Zhao, Youjie; Chen, Yuan; Xiong, Zhi; Wang, Liuyang; Tian, Bin
	2015
发表期刊	EVOLUTIONARY BIOINFORMATICS
卷号	11 页码:27-37
摘要	High-throughput transcriptome provides an unbiased approach for understanding the genetic basis and gene functions in response to different conditions. Here we sequenced RNA-seq libraries derived from a Bombax ceiba L. system under a controlled experiment. As a known medicinal and ornamental plant, B. ceiba grows mainly in hot-dry monsoon rainforests in Southeast Asia and Australia. Due to the specific growth environment, it has evolved a unique system that enables a physiologic response to drought stress. To date, few studies have characterized the genome-wide features of drought endurance in B. ceiba. In this study, we first attempted to characterize and identify the most differentially expressed genes and associated functional pathways under drought treatment and normal condition. Using RNA-seq technology, we generated the first transcriptome of B. ceiba and identified 59 differentially expressed genes with greater than 1,000-fold changes under two conditions. The set of upregulated genes implicates interplay among various pathways: plants growth, ubiquitin-mediated proteolysis, polysaccharides hydrolyzation, oxidative phosphorylation and photosynthesis, etc. In contrast, genes associated with stem growth, cell division, fruit ripening senescence, disease resistance, and proline synthesis are repressed. Notably, key genes of high RPKM levels in drought are AUX1, JAZ, and psbS, which are known to regulate the growth of plants, the resistance against abiotic stress, and the photosynthesis process. Furthermore, 16,656 microsatellite markers and 3,071 single-nucleotide polymorphisms (SNPs) were predicted by in silico methods. The identification and functional annotation of differentially expressed genes, microsatellites, and SNPs represent a major step forward and would serve as a valuable resource for understanding the complexity underlying drought endurance and adaptation in B. ceiba.
关键词	Transcriptome Bombax Ceiba Rna-seq Drought Endurance
DOI	10.4137/EBO.S20620
收录类别	SCI
语种	英语
WOS记录号	WOS:000366735800003
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/25313
专题	中国科学院东亚植物多样性与生物地理学重点实验室
推荐引用方式 GB/T 7714	Zhou, Zhili,Ma, Huancheng,Lin, Kevin,et al. RNA-seq Reveals Complicated Transcriptomic Responses to Drought Stress in a Nonmodel Tropic Plant, Bombax ceiba L.[J]. EVOLUTIONARY BIOINFORMATICS,2015,11:27-37.
APA	Zhou, Zhili.,Ma, Huancheng.,Lin, Kevin.,Zhao, Youjie.,Chen, Yuan.,...&Tian, Bin.(2015).RNA-seq Reveals Complicated Transcriptomic Responses to Drought Stress in a Nonmodel Tropic Plant, Bombax ceiba L..EVOLUTIONARY BIOINFORMATICS,11,27-37.
MLA	Zhou, Zhili,et al."RNA-seq Reveals Complicated Transcriptomic Responses to Drought Stress in a Nonmodel Tropic Plant, Bombax ceiba L.".EVOLUTIONARY BIOINFORMATICS 11(2015):27-37.

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