Production of N-15-labeled alpha-amanitin in Galerina marginata

doi:10.1016/j.toxicon.2015.06.018

	Production of N-15-labeled alpha-amanitin in Galerina marginata
	Luo,Hong; DuBois,Brandon; Sgambelluri,R. Michael; Angelos,Evan R.; Li,Xuan; Holmes,Daniel; Walton,Jonathan D.
	2015-09-01
发表期刊	TOXICON
卷号	103 页码:60-64
摘要	alpha-Amanitin is the major causal constituent of deadly Amanita mushrooms that account for the majority of fatal mushroom poisonings worldwide. It is also an important biochemical tool for the study of its target, RNA polymerase II. The commercial supply of this bicyclic peptide comes from Amanita phalloides, the death cap mushroom, which is collected from the wild. Isotopically labeled amanitin could be useful for clinical and forensic applications, but alpha-amanitin has not been chemically synthesized and A. phalloides cannot be cultured on artificial medium. Using Galerina marginata, an unrelated saprotrophic mushroom that grows and produces alpha-amanitin in culture, we describe a method for producing N-15-labeled alpha-amanitin using growth media containing N-15 as sole nitrogen source. A key to success was preparing N-15-enriched yeast extract via a novel method designated "glass bead-assisted maturation." In the presence of the labeled yeast extract and 15N NH4Cl, alpha-amanitin was produced with >97% isotope enrichment. The labeled product was confirmed by HPLC, high-resolution mass spectrometry, and NMR. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Amanita Galerina Alpha-amanitin Isotope Labeling Lc-ms Nmr
DOI	10.1016/j.toxicon.2015.06.018
收录类别	SCI
语种	英语
WOS记录号	WOS:000359888300008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/25107
专题	中国科学院东亚植物多样性与生物地理学重点实验室
推荐引用方式 GB/T 7714	Luo,Hong,DuBois,Brandon,Sgambelluri,R. Michael,et al. Production of N-15-labeled alpha-amanitin in Galerina marginata[J]. TOXICON,2015,103:60-64.
APA	Luo,Hong.,DuBois,Brandon.,Sgambelluri,R. Michael.,Angelos,Evan R..,Li,Xuan.,...&Walton,Jonathan D..(2015).Production of N-15-labeled alpha-amanitin in Galerina marginata.TOXICON,103,60-64.
MLA	Luo,Hong,et al."Production of N-15-labeled alpha-amanitin in Galerina marginata".TOXICON 103(2015):60-64.

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