Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds

doi:10.1111/j.1365-3040.2009.02053.x

	Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds
	Roach, Thomas 1,2; Beckett, Richard P.3; Minibayeva, Farida V.4; Colville, Louise 1; Whitaker, Claire 3; Chen, Hongying 1,5; Bailly, Christophe 6; Kranner, Ilse 1
	2010
发表期刊	PLANT CELL AND ENVIRONMENT
卷号	33 期号:1 页码:59-75
摘要	Reactive oxygen species (ROS) are implicated in seed death following dehydration in desiccation-intolerant 'recalcitrant' seeds. However, it is unknown if and how ROS are produced in the apoplast and if they play a role in stress signalling during desiccation. We studied intracellular damage and extracellular superoxide (O-2 center dot-) production upon desiccation in Castanea sativa seeds, mechanisms of O-2 center dot- production and the effect of exogenously supplied ROS. A transient increase in extracellular O-2 center dot- production by the embryonic axes preceded significant desiccation-induced viability loss. Thereafter, progressively more oxidizing intracellular conditions, as indicated by a significant shift in glutathione half-cell reduction potential, accompanied cell and axis death, coinciding with the disruption of nuclear membranes. Most hydrogen peroxide (H2O2)-dependent O-2 center dot- production was found in a cell wall fraction that contained extracellular peroxidases (ECPOX) with molecular masses of similar to 50 kDa. Cinnamic acid was identified as a potential reductant required for ECPOX-mediated O-2 center dot- production. H2O2, applied exogenously to mimic the transient ROS burst at the onset of desiccation, counteracted viability loss of sub-lethally desiccation-stressed seeds and of excised embryonic axes grown in tissue culture. Hence, extracellular ROS produced by embryonic axes appear to be important signalling components involved in wound response, regeneration and growth.
关键词	Castanea Sativa Germination Glutathione Hydrogen Peroxide Peroxidase Phenolic Acid Reactive Oxygen Species Seed Superoxide
DOI	10.1111/j.1365-3040.2009.02053.x
收录类别	SCI
语种	英语
WOS记录号	WOS:000272661000006
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/23910
专题	中国西南野生生物种质资源库
作者单位	1.Royal Bot Gardens Kew, Seed Conservat Dept, Ardingly RH17 6TN, W Sussex, England 2.UCL, Res Dept Genet Evolut & Environm, London WC1E 6BT, England 3.Univ KwaZulu Natal, Sch Biol & Conservat Sci, ZA-3209 Pietermaritzburg, Scottsville, South Africa 4.Russian Acad Sci, Kazan Inst Biochem & Biophys, Kazan 420111, Russia 5.Chinese Acad Sci, Kunming Inst Bot, Kunming 661100, Peoples R China 6.Univ Paris 06, EA2388, F-75005 Paris, France
推荐引用方式 GB/T 7714	Roach, Thomas,Beckett, Richard P.,Minibayeva, Farida V.,et al. Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds[J]. PLANT CELL AND ENVIRONMENT,2010,33(1):59-75.
APA	Roach, Thomas.,Beckett, Richard P..,Minibayeva, Farida V..,Colville, Louise.,Whitaker, Claire.,...&Kranner, Ilse.(2010).Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds.PLANT CELL AND ENVIRONMENT,33(1),59-75.
MLA	Roach, Thomas,et al."Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds".PLANT CELL AND ENVIRONMENT 33.1(2010):59-75.

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