Connecting Mutations of the RNA Polymerase II C-Terminal Domain to Complex Phenotypic Changes Using Combined Gene Expression and Network Analyses

doi:10.1371/journal.pone.0011386

	Connecting Mutations of the RNA Polymerase II C-Terminal Domain to Complex Phenotypic Changes Using Combined Gene Expression and Network Analyses
	Rogers, Carlyle 1; Guo, Zhenhua2 ; Stiller, John W.1
	2010-06-30
发表期刊	PLOS ONE
卷号	5 期号:6 页码:e11386
摘要	The C-terminal domain (CTD) of the largest subunit in DNA-dependent RNA polymerase II (RNAP II) is essential for mRNA synthesis and processing, through coordination of an astounding array of protein-protein interactions. Not surprisingly, CTD mutations can have complex, pleiotropic impacts on phenotype. For example, insertions of five alanine residues between CTD diheptads in yeast, which alter the CTD's overall tandem structure and physically separate core functional units, dramatically reduce growth rate and result in abnormally large cells that accumulate increased DNA content over time. Patterns by which specific CTD-protein interactions are disrupted by changes in CTD structure, as well as how downstream metabolic pathways are impacted, are difficult to target for direct experimental analyses. In an effort to connect an altered CTD to complex but quantifiable phenotypic changes, we applied network analyses of genes that are differentially expressed in our five alanine CTD mutant, combined with established genetic interactions from the Saccharomyces cerevisiae Genome Database (SGD). We were able to identify candidate genetic pathways, and several key genes, that could explain how this change in CTD structure leads to the specific phenotypic changes observed. These hypothetical networks identify links between CTD-associated proteins and mitotic function, control of cell cycle checkpoint mechanisms, and expression of cell wall and membrane components. Such results can help to direct future genetic and biochemical investigations that tie together the complex impacts of the CTD on global cellular metabolism.
DOI	10.1371/journal.pone.0011386
收录类别	SCI
语种	英语
WOS记录号	WOS:000279370000015
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/23903
专题	中国西南野生生物种质资源库
作者单位	1.E Carolina Univ, Dept Biol, Greenville, NC 27858 USA 2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Rogers, Carlyle,Guo, Zhenhua,Stiller, John W.. Connecting Mutations of the RNA Polymerase II C-Terminal Domain to Complex Phenotypic Changes Using Combined Gene Expression and Network Analyses[J]. PLOS ONE,2010,5(6):e11386.
APA	Rogers, Carlyle,Guo, Zhenhua,&Stiller, John W..(2010).Connecting Mutations of the RNA Polymerase II C-Terminal Domain to Complex Phenotypic Changes Using Combined Gene Expression and Network Analyses.PLOS ONE,5(6),e11386.
MLA	Rogers, Carlyle,et al."Connecting Mutations of the RNA Polymerase II C-Terminal Domain to Complex Phenotypic Changes Using Combined Gene Expression and Network Analyses".PLOS ONE 5.6(2010):e11386.

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