Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization
Hu, Xiangyang1,2,3; Kong, Xiangxiang1,2,3; Wang, Chuntao1,2,3; Ma, Lan1,2,4; Zhao, Jinjie1,2,4; Wei, Jingjing1,2,4; Zhang, Xiaoming1,2,4; Loake, Gary J.5; Zhang, Ticao1; Huang, Jinling1,6; Yang, Yongping1,2,3
2014-12-01
Source PublicationPLANT CELL
Volume26Issue:12Pages:4763-4781
AbstractWinter-annual accessions of Arabidopsis thaliana require either exposure to cold stress or vernalization to initiate flowering via FRIGIDA (FRI). FRI acts as a scaffold protein to recruit several chromatin modifiers that epigenetically modify flowering genes. Here, we report that proteasome-mediated FRI degradation regulates flowering during vernalization in Arabidopsis. Our genetic and biochemical experiments demonstrate that FRI directly interacts with the BTB (Bric-a-Brac/Tramtrack/Broad Complex) proteins LIGHT-RESPONSE BTB1 (LRB1) and LRB2 as well as the CULLIN3A (CUL3A) ubiquitin-E3 ligase in vitro and in vivo, leading to proteasomal degradation of FRI during vernalization. The degradation of FRI is accompanied by an increase in the levels of the long noncoding RNA ColdAIR, which reduces the level of histone H3Lys4 trimethylation (H3K4me3) in FLOWERING LOCUS C chromatin to promote flowering. Furthermore, we found that the cold-induced WRKY34 transcription factor binds to the W-box in the promoter region of CUL3A to modulate CUL3A expression. Deficiency of WRKY34 suppressed CUL3A transcription to enhance FRI protein stability and led to late flowering after vernalization. Conversely, overexpression of WRK34 promoted FRI degradation and early flowering through inducing CUL3A accumulation. Together, these data suggest that WRKY34-induced and CUL3A-dependent proteolysis of FRI modulate flowering in response to vernalization.
DOI10.1105/tpc.114.132738
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS IDWOS:000348646000018
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/20466
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Inst Tibetan Plateau Res, Lab Alpine Ecol & Biodivers, Beijing 100101, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3JR, Midlothian, Scotland
6.E Carolina Univ, Dept Biol, Greenville, NC 27858 USA
Recommended Citation
GB/T 7714
Hu, Xiangyang,Kong, Xiangxiang,Wang, Chuntao,et al. Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization[J]. PLANT CELL,2014,26(12):4763-4781.
APA Hu, Xiangyang.,Kong, Xiangxiang.,Wang, Chuntao.,Ma, Lan.,Zhao, Jinjie.,...&Yang, Yongping.(2014).Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization.PLANT CELL,26(12),4763-4781.
MLA Hu, Xiangyang,et al."Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization".PLANT CELL 26.12(2014):4763-4781.
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