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题名: CRYOPRESERVATION OF ADVENTITIOUS SHOOT TIPS OF PARAISOMETRUM MILEENSE BY DROPLET VITRIFICATION
作者: Lin, Liang1, 2; Yuan, Bin1; Wang, Dandan1; Lin, Weiqi1
刊名: CRYOLETTERS
关键词: Gesneriaceae ; endangered species ; sucrose preculture ; cold acclimation
英文摘要: BACKGROUND: Gesneriaceae family contains numerous species endemic to China, and many of them are listed as endangered species. There is a need for a simple and efficient method for long term conservation of these species. OBJECTIVE: The study aimed to establish an efficient procedure for cryopreserving Paraisometrum mileense, a critically endangered species endemic to Yunnan, China. METHODS: Effects of sucrose concentration of preculture solution, duration of sucrose preculture, duration of plant vitrification solution 2 (PVS2) treatment, and cold acclimation on regeneration of cryopreserved adventitious shoot tips (ASTs) were assessed. RESULTS AND CONCLUSION: Among different sucrose preculture regimes tested, preculture with 0.3M sucrose for 24h resulted in best regeneration of cryopreserved ASTs. PVS2 treatment also affected regeneration considerably with the maximum survival of ASTs after incubation in PVS2 for 90 min at 0 degrees C. With the optimised parameters, the level of shoot regeneration from cryopreserved ASTs reached 86%. No morphological abnormalities were observed during one year's growth of the plantlets developing from cryopreserved ASTs. Procedure established in this research is a promising technique for the cryopreservation of ASTs of this species.
出版日期: 2014
卷号: 35, 期号:1, 页码:22-28
语种: 英语
收录类别: SCI
ISSN号: 0143-2044
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18224
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog & Germplasm Bank Wild, Kunming, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Lin,Liang;Yuan,Bin;Wang,Dandan;Lin,Weiqi.CRYOPRESERVATION OF ADVENTITIOUS SHOOT TIPS OF PARAISOMETRUM MILEENSE BY DROPLET VITRIFICATION,CRYOLETTERS,2014,35(1):22-28
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