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Quantitative Proteomics Analysis Reveals That the Nuclear Cap-Binding Complex Proteins Arabidopsis CBP20 and CBP80 Modulate the Salt Stress Response
Kong, Xiangxiang1,2,3,4; Ma, Lan1,2,3,4; Yang, Liming5; Chen, Qian1,2,3; Xiang, Nan1,2,3; Yang, Yongping1,2,3,5; Hu, Xiangyang1,2,3,5; Hu,XY (reprint author),Chinese Acad Sci,Key Lab Plant Divers & Biogeog East Asia,132 Lanhei Rd,Heilongtan 650204,Kunming,Peoples R China.; huxiangyang@mail.kib.ac.cn
2014-05-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume13Issue:5Pages:2495-2510
AbstractThe cap-binding proteins CBP20 and CBP80 have well-established roles in RNA metabolism and plant growth and development. Although these proteins are thought to be involved in the plant's response to environmental stress, their functions in this process are unclear. Here we demonstrated that Arabidopsis cbp20 and cbp80 null mutants had abnormal leaves and flowers and exhibited increased sensitivity to salt stress. The aberrant phenotypes were more pronounced in the cbp20/80 double mutant. Quantification by iTRAQ (isobaric tags for relative and absolute quantification) identified 77 differentially expressed proteins in the cbp20 and cbp80 lines compared with the wild-type Col-0 under salt stress conditions. Most of these differentially expressed proteins were synergistically expressed in cbp20 and cbp80, suggesting that CBP20 and CBP80 have synergistic roles during the salt stress response. Biochemical analysis demonstrated that CBP20 and CBP80 physically interacted with each other. Further analysis revealed that CBP20/80 regulated the splicing of genes involved in proline and sugar metabolism and that the epigenetic and post-translational modifications of these genes were involved in salt stress tolerance. Our data suggest a link between CBP20/80-dependent protein ubiquitination/sumoylation and the salt stress response.
KeywordArabidopsis Cbp20 Cbp80 Itraq
Subject AreaBiochemistry & Molecular Biology
DOI10.1021/pr4012624
Indexed BySCI
Language英语
WOS IDWOS:000335490600023
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18166
Collection中国科学院青藏高原研究所昆明部
Corresponding AuthorHu,XY (reprint author),Chinese Acad Sci,Key Lab Plant Divers & Biogeog East Asia,132 Lanhei Rd,Heilongtan 650204,Kunming,Peoples R China.; huxiangyang@mail.kib.ac.cn
Affiliation1.Chinese Acad Sci, Key Lab Plant Divers & Biogeog East Asia, Heilongtan 650204, Kunming, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Heilongtan 650204, Kunming, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Heilongtan 650204, Kunming, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Huaiyin Normal Univ, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Sch Life Sci, Huaian 223300, Peoples R China
Recommended Citation
GB/T 7714
Kong, Xiangxiang,Ma, Lan,Yang, Liming,et al. Quantitative Proteomics Analysis Reveals That the Nuclear Cap-Binding Complex Proteins Arabidopsis CBP20 and CBP80 Modulate the Salt Stress Response[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(5):2495-2510.
APA Kong, Xiangxiang.,Ma, Lan.,Yang, Liming.,Chen, Qian.,Xiang, Nan.,...&huxiangyang@mail.kib.ac.cn.(2014).Quantitative Proteomics Analysis Reveals That the Nuclear Cap-Binding Complex Proteins Arabidopsis CBP20 and CBP80 Modulate the Salt Stress Response.JOURNAL OF PROTEOME RESEARCH,13(5),2495-2510.
MLA Kong, Xiangxiang,et al."Quantitative Proteomics Analysis Reveals That the Nuclear Cap-Binding Complex Proteins Arabidopsis CBP20 and CBP80 Modulate the Salt Stress Response".JOURNAL OF PROTEOME RESEARCH 13.5(2014):2495-2510.
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