Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings

	Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings
	Deng, Wei-Wei 1,2; Fei, Yue 1,2; Wang, Shuo 1,2; Wan, Xiao-Chun 1,2; Zhang, Zheng-Zhu 1,2; Hu, Xiang-Yang 3
通讯作者	Zhang,ZZ (reprint author),Anhui Agr Univ,Key Lab Tea Biochem & Biotechnol,Minist Educ,130 Changjiang West Rd,Hefei 230036,Anhui,Peoples R China.,zzz@ahau.edu.cn
	2013-12-01
发表期刊	PLANT GROWTH REGULATION
ISSN	0167-6903
卷号	71 期号:3 页码:295-299
摘要	Theanine synthetase (TS) is a key enzyme involved in theanine biosynthesis. In our recent study, it has been revealed that theanine biosynthesis derived from nitrogen metabolism in tea (Camellia sinensis) plants can be influenced by shading treatment. The expression patterns of CsTS protein in the roots and shoots of tea seedlings were examined by western blot using a self-prepared polyclonal antibody with high specificity and sensitivity. The effect of long-term shade treatment on the levels of theanine synthesis was also investigated using roots and shoots of tea seedlings. Levels of theanine and total free amino acids gradually increased in shoots, reaching their maximum after 22 days of treatment (DOT). The immunoblotting analysis suggested that CsTS protein levels increased gradually up to 22 DOT and expression remained at a high level, except after 1 DOT where levels were low in both roots and shoots. The increased theanine concentration we observed in the shading treatment may be due to increased nitrogen assimilation and reduced theanine catabolism under shade conditions.
关键词	Camellia Sinensis Theanine Synthetase Shade Theanine Amino Acid
学科领域	Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000326622700010
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/17430
专题	中国科学院青藏高原研究所昆明部
作者单位	1.Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, Hefei 230036, Anhui, Peoples R China 2.Anhui Agr Univ, Minist Agr, Hefei 230036, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Inst Tibet Plateau Res Kunming, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Deng, Wei-Wei,Fei, Yue,Wang, Shuo,et al. Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings[J]. PLANT GROWTH REGULATION,2013,71(3):295-299.
APA	Deng, Wei-Wei,Fei, Yue,Wang, Shuo,Wan, Xiao-Chun,Zhang, Zheng-Zhu,&Hu, Xiang-Yang.(2013).Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings.PLANT GROWTH REGULATION,71(3),295-299.
MLA	Deng, Wei-Wei,et al."Effect of shade treatment on theanine biosynthesis in Camellia sinensis seedlings".PLANT GROWTH REGULATION 71.3(2013):295-299.

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