Comparative Proteome Analyses Reveal that Nitric Oxide Is an Important Signal Molecule in the Response of Rice to Aluminum Toxicity

doi:10.1021/pr300971n

	Comparative Proteome Analyses Reveal that Nitric Oxide Is an Important Signal Molecule in the Response of Rice to Aluminum Toxicity
	Yang, Liming 1; Tian, Dagang 2,3; Todd, Christopher D.4; Luo, Yuming 1; Hu, Xiangyang 2
通讯作者	Luo, YM (reprint author), Huaiyin Normal Univ, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Sch Life Sci, Huaian 223300, Peoples R China. ; yuming@163.com ; huxiangyang@mail.kib.ac.cn
	2013-03-01
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	12 期号:3 页码:1316-1330
摘要	Acidic soils inhibit crop yield and reduce grain quality. One of the major contributing factors to acidic soil is the presence of soluble aluminum (Al3+) ions, but the mechanisms underlying plant responses to Al3+ toxicity remain elusive. Nitric oxide (NO) is an important messenger and participates in various plant physiological responses. Here, we demonstrate that Al3+ induced an increase of NO in rice seedlings; adding exogenous NO alleviated the Al3+ toxicity related to rice growth and photosynthetic capacity, effects that could be reversed by suppressing NO metabolism. Comparative proteomic analyses successfully identified 92 proteins that showed differential expression after Al3+ or NO treatment. In particular, some of the proteins are involved in reactive oxygen species (ROS) and reactive nitrogen species (RNS) metabolism. Further analyses confirmed that NO treatment reduced Al3+-induced ROS and RNS toxicities by increasing the activities and protein expression of antioxidant enzymes, as well as S-nitrosoglutathione reductase (GSNOR). Suppressing GSNOR enzymatic activity aggravated Al3+ damage to rice and increased the accumulation of RNS. NO treatment altered the expression of proteins associated with cell wall synthesis, cell division and cell structure, calcium signaling and defense responses. On the basis of these results, we propose that NO activates multiple pathways that enhance rice adaptation to Al3+ toxicity. Such findings may be applicable to crop engineering to enhance yield and improve stress tolerance.
关键词	Aluminum Nitric Oxide S-nitrosoglutathione Reductase Reactive Nitrogen Species Reactive Oxygen Species
学科领域	Biochemistry & Molecular Biology
DOI	10.1021/pr300971n
收录类别	SCI
语种	英语
WOS记录号	WOS:000315708000023
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/16515
专题	中国科学院青藏高原研究所昆明部
作者单位	1.Huaiyin Normal Univ, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Sch Life Sci, Huaian 223300, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 3.Fujian Acad Agr Sci, Inst Biotechnol, Fujian Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China 4.Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada
推荐引用方式 GB/T 7714	Yang, Liming,Tian, Dagang,Todd, Christopher D.,et al. Comparative Proteome Analyses Reveal that Nitric Oxide Is an Important Signal Molecule in the Response of Rice to Aluminum Toxicity[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(3):1316-1330.
APA	Yang, Liming,Tian, Dagang,Todd, Christopher D.,Luo, Yuming,&Hu, Xiangyang.(2013).Comparative Proteome Analyses Reveal that Nitric Oxide Is an Important Signal Molecule in the Response of Rice to Aluminum Toxicity.JOURNAL OF PROTEOME RESEARCH,12(3),1316-1330.
MLA	Yang, Liming,et al."Comparative Proteome Analyses Reveal that Nitric Oxide Is an Important Signal Molecule in the Response of Rice to Aluminum Toxicity".JOURNAL OF PROTEOME RESEARCH 12.3(2013):1316-1330.

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