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珠芽摩芋种苗繁育方法
龙春林 ; 程治英 ; 罗吉凤
Application Number200410040723
Rights Holder龙春林
Date Available2005-03-23
Abstract一种珠芽摩芋种苗繁育方法,利用组织培养技术繁殖珠芽摩芋,先取珠芽魔芋的珠芽或叶片或叶柄或根或块茎芽即嫩芽或鳞叶或块茎切片,在MS或1/2MS或N6基本培养基中附加0-5mg.L-1的植物激素BA或Kt或植物激素组合BA+NAA或BA+IAA或Kt+NAA或Kt+IAA,蔗糖浓度3%,pH值=5.8,光照时间12h·d-1,光照强度1000-1500lx,培养温度为24±1℃下培养,然后将以上各外植体产生的愈伤组织和不定芽组织块移入MS+BA 0.5+NAA0.2的培养基中继续生长,形成不规则状结构后切割继代,再将试管芽丛转入仅含生长素的1/2MS培养基上进行生根培养,20天后,将试管苗直接移入没经特殊处理的基质中即可。
Document Type专利
Identifierhttp://ir.kib.ac.cn/handle/151853/11009
Collection资源植物与生物技术所级重点实验室
Recommended Citation
GB/T 7714
龙春林,程治英,罗吉凤. 珠芽摩芋种苗繁育方法. CN200410040723.0.
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