宏基因组文库的保存和筛选方法
曾英 ; 付小莉 ; 王浩鑫.
Application Number201010297594.9
Rights Holder曾英
Date Available2011-02-09
Abstract提供一种宏基因组文库的保存和筛选方法,一是快速有效地保存海量宏基因组克隆的方法,包括用小量的文库包装颗感染一定量的感受态细胞,等分成96份或96的整倍数,进行文库扩增培养与保存;二是通过对同一张膜上的文库DNA模板用不同探针进行多次杂交以提高宏基因组文库筛选效率的方法,包括用一定温度的碱性溶液去除杂交膜上的探针,同时保留膜上的DNA模板用于不同探针的多次膜杂交,从而提高宏基因组文库的筛选效率。
Document Type专利
Identifierhttp://ir.kib.ac.cn/handle/151853/10597
Collection植物化学与西部植物资源持续利用国家重点实验室
Recommended Citation
GB/T 7714
曾英,付小莉,王浩鑫.. 宏基因组文库的保存和筛选方法. 201010297594.9.
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201112300021.pdf(288KB) 开放获取--Application Full Text
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